![]() ![]() ![]() Two bases are complementary if they form Watson-Crick base pairs. In molecular biology, complementarity is a property shared between two nucleic acid sequences, such that when they are aligned antiparallel to each other, the nucleotide bases at each position will be complementary. The suggested siRNAs can be rescanned against additional sequences which is very useful in designing siRNAs against the same gene for different organisms.Ĭalculate the molecular weight and determine the required mass or volume to obtain solutions of a specific concentration.Ĭopyright © 2000-2020 CLOCS All rights reserved. DNA reverse and complementary sequence generator. Compatible with wild type or mutated aequorin and with normal or N-type coelenterizine.Ĭalculate the concentration of dsDNA, ssDNA, or RNA from absorbance values.ĭesign siRNAs for any specified DNA/RNA sequence. This calculator provides instructions on how to dilute a DNA stock solution to obtain specific DNA copy number per L. With this DNA reverse and complementary sequence generator online tool, the user can convert the RNA or DNA sequence into: Reverse, complementary and. The pairing of nucleotides within the DNA double-helix is complementary which. single strands acts as a template for a new strand of complementary DNA. A DNA sequence whose 5-to-3 sequence is identical on each DNA strand. Features include restriction digests, translation, complement, reverse complement, and more.Ĭalculate intracellular calcium concentration with aequorin bioluminescence. The Central Dogma of Molecular Biology states that DNA makes RNA makes proteins. Slice, dice, and splice any nucleotide sequence. Reverse Complement Reverse Complement converts a DNA sequence into its reverse, complement, or reverse-complement counterpart. ![]()
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